Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Concomitant differentiation of a population of mouse embryonic stem cells into neuron-like cells and Schwann cell-like cells in a slow-flow microfluidic device

doi: 10.1002/dvdy.24466

Figure Lengend Snippet: RTqPCR quantification of gene marker expression in the differentiated neuron-like cells (a). OCT4 expression in undifferentiated mES cells and neuron-like cells differentiated from mES cells. D3: Undifferentiated condition, statoacoustic ganglion: (SAG) spiral ganglion neuron culture (control). (b). Expression of proneural marker genes in neuron-like cells cells. Ngn1, NeuroD and Nfil expression is much higher in neuronally differentiated cells, but not detected in undifferentiated condition (D3ES). (c). Expression of mature neuronal marker genes: TrkB, TrkC, Prph in the neuron-like cells are upregulated in all the neuronal differentiated conditions and expression levels are much higher under conditions with DHA addition. F12: Neuronal basal media, C: CNTF differentiation condition, N: NGF differentiation condition, M: MIF differentiation condition, D: DHA differentiation condition, CD: CNTF+DHA differentiation condition, ND: NGF+DHA differentiation condition, MD: MIF+DHA differentiation condition. (d). Expression of auditory neuronal markers, GabaAR, VgluT1 and SP19 are highly upregulated in the neuronal differentiation conditions compared to undifferentiated conditions and F12 (basal) medium. VgluT1 expression is much higher in the MIF+DHA condition than all the other differentiation conditions, which was to be expected, given that MIF is the inner ear’s first differentiation “neurotrophin” (Holmes et al., 2011; Bank et al. 2012; Shen et al., 2012).

Article Snippet: Culturing mES cells Cell lines: The cell line used for these studies was the D3 mES cell line (ATCC-CRL-1934; Doetschman et al., 1985 ).

Techniques: Marker, Expressing, Control